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KMID : 0545120130230060837
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Journal of Microbiology and Biotechnology
2013 Volume.23 No. 6 p.837 ~ p.842
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Isolation and Characterization of a Cryptic Plasmid, pMBLR00, from Leuconostoc mesenteroides subsp. mesenteroides KCTC 3733
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Chae Han-Seung
Lee Jeong-Min
Lee Ju-Hoon
Lee Pyung-Cheon
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Abstract
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A cryptic plasmid, pMBLR00, from Leuconostoc mesenteroides subsp. mesenteroides KCTC 3733 was isolated, characterized, and used for the construction of a cloning vector to engineer Leuconostoc species. pMBLR00 is a rolling circle replication plasmid, containing 3,370 base pairs. Sequence analysis revealed that pMBLR00 has 3 open reading frames: Cop (copy number control protein), Rep (replication protein), and Mob (mobilization protein). pMBLR00 replicates by rolling circle replication, which was confirmed by the presence of a conserved double-stranded origin and single-stranded DNA intermediates. An Escherichia coli-Leuconostoc shuttle vector, pMBLR02, was constructed and was able to replicate in Leuconostoc citreum 95. pMBLR02 could be a useful genetic tool for metabolic engineering and the genetic study of Leuconostoc species.
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KEYWORD
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cryptic plasmid, Leuconostoc mesenteroides , rolling circle replication mechanism, D-lactic acid
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